| Accredited study performed by the Biological Dosimetry Laboratory – analysis of dicentric chromosome frequencies. AB 1577 Determination of absorbed dose from 60Co gamma radiation and 200 keV X-rays based on the assessment of the frequency of dicentric chromosomes in human peripheral blood lymphocytes. Method range: 0.2 Gy - 4 Gy. Cytogenetic biological dosimetry method. The current scope of accreditation and additional information about the Biological Dosimetry Laboratory can be found on the Polish Center for Accreditation website: https://www.pca.gov.pl/akredytowane-podmioty/akredytacje-aktywne/laboratoria-badawcze/AB%201577,podmiot.html The cost of a blood sample test is: PLN 3,000 + 23% VAT |
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Biological Dosimetry Laboratory
Institute of Nuclear Chemistry and Technology
ul. Dorodna 16
03-195 Warsaw
Poland
Tel.: (+4822) 5041220, 5041000
Fax: (+4822) 8111917, 8111532
Head of the Biological Dosimetry Laboratory – Dr. Sylwester Sommer (Tel. (+4822) 5041228, mobile: 508536773, e-mail: s.sommer@ichtj.waw.pl or silver.sommer@poczta.fm)
Deputy Head of the Biological Dosimetry Laboratory for Technical Affairs – M.Sc. Eng. Iwona Buraczewska (tel. (+4822) 5041226, e-mail: i.buraczewska@ichtj.waw.pl)
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| Method description (not accredited): The dicentric chromosome frequency test in peripheral blood lymphocytes allows for the assessment of a given individual's radiation-absorbed dose. Lymphocytes in a collected blood sample are stimulated to divide, cultured for 24 hours, and then fixed and placed on microscope slides. The frequency of dicentric chromosomes in unirradiated control blood samples is in the range of 1 dicentric chromosome per 1000–2000 cells in the first metaphase after division. Dicentric chromosomes are aberrations induced specifically by ionizing radiation and by certain cytostatic drugs with radiation-like effects (radiomimetics). The absorbed dose to the whole body (received over a short period of time – acute dose) is estimated using the calibration curve for cobalt-60 or X-rays (200 kV, 4.5 mA at 200 keV), and a 95% confidence interval is determined. Using standard statistical methods, it is possible to convert the absorbed dose over a short period of time to a chronic dose if it was received over several dozen hours, and to estimate whether the dose was administered to the whole body or only to a portion of it. All calculations are based on the methods included in the ISO 19238:2014 standard. Figure: A human lymphocyte in mitosis. Dicentric chromosomes are marked with red arrows. The centric ring and the accompanying dicentric and acentric fragments are marked with black and blue arrows. |
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